attune flow cytometer - An Overview

attune flow cytometer - An Overview

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Details-enriched characterization of cell populations. Morphological data from illustrations or photos can include to the richness of flow cytometry facts. For instance, the figure shows an usually common apoptosis assay applying Annexin V and PI, incorporating cell imaging to characterize cells in each populace to expose morphologically unique capabilities. These insights could not are already obtained from multiplex staining on your own.

Whether or not you’re a starter or a specialist, explore our assets to advance your exploration and know-how in flow cytometry.

(B) Uncooked 264.seven macrophages in many cases are annotated inaccurately with regard to particle rely because of their complexity and noncircular borders. The number of singlets accessible for Evaluation increases after the user trains and employs a far more exact design.

Discover new insights into your experimental data by visualizing populations employing mixed fluorescence and morphology details. Our program interface wizards allow downsampling, concatenation (merged data files) and dimensionality equipment which can be developed into the software package for an intuitive, streamlined knowledge analysis practical experience.

*Pricing is indicative only which is issue to alter. Be sure to Get in touch with your Agilent representative with any inquiries. The knowledge set forth herein is not really a suggestion or maybe a commitment, both equally of which can be issue to credit rating acceptance, terms and conditions, and availability with Agilent. Agilent Money Remedies is available in chosen countries.

Below, a highly trained user has gated singlets confidently. After assessing the guide singlet gate, the CytPix impression-derived parameter ParticleCount reveals this gate consists of greater than 4% aggregates.

Pair your Attune flow cytometer using an autosampler for increased workflow effectiveness, and increase robotic integration for max throughput.

Steady impression good quality even at substantial throughput Acoustic focusing plus a significant-pace camera Mix to image these T cells regularly at small or significant flow premiums. Easily change aim and camera options to fulfill experimental specifications.

Early signs of this contamination had at first been dismissed as debris. Backgating to find new populations. Backgating imaged cells over the Attune CytPix Flow Cytometer also enables you to use morphological features to find out attention-grabbing subpopulations that will not be obvious from flow cytometry knowledge by itself.

Most likely most of all, these situations comprise cells of Plainly different phenotypes which could lead to incorrect conclusions about double website good situations (specifically in scarce populations).

AI-driven automated picture Examination With all the Attune Cytometric Software package translates event capabilities into unique morphology parameters that may be combined with normal fluorescence and scatter parameters to reveal hugely reproducible population variations and distinctive subsets.

Software Take note: Automated picture Evaluation decreases person-to-user variability in flow cytometry gating approaches

Leverage the power of advanced technological know-how and innovation to accelerate your discoveries. Rely on inside the Attune Flow Cytometer family to provide the dependability, velocity, and precision that set your get the job done apart.

No wash/no lyse leukocyte Examination with imaging. Cells ended up acquired from 24-hour-previous blood by dilution in 1 mM EDTA ( Expert UX/UI permits people to train AI designs for expanded purposes. (A) The “Teach Model” function gives annotation equipment and authentic-time model training accuracy.

At the center of every Attune Flow Cytometer lies our groundbreaking acoustic concentrating technological know-how, which makes use of audio waves to meticulously align cells inside the flow mobile. This ground breaking approach surpasses standard hydrodynamic solutions, leading to faster processing speeds, Extraordinary information good quality, and greater sample throughput.

As the cell line experienced Beforehand been analyzed within the Attune CytPix Flow Cytometer, the researchers went back again to the pictures and were being able to document the microbial an infection at least five days previously.

The Attune CytPix Flow Cytometer makes it possible for customers to visualise samples throughout acquisition, validate the precision of gating tactics, and help final results with responsible documentation for publication applications.

Listed here, we show that by gating approaches utilizing picture-centered quantitative parameters can distinguish interacting cells from coincident gatherings to additional properly review interacting cells.